Journal: Pharmaceuticals

Article Title: Anti-Inflammatory Effects of Aptamin C in Pulmonary Fibrosis Induced by Bleomycin

doi: 10.3390/ph17121577

Figure Lengend Snippet: Protective effect of Aptamin C on bleomycin-induced mortality in mice. Survival rates were measured in mice ( n = 8) subjected to intratracheal injection of bleomycin (1.5 mg/kg) or PBS alone (negative control (NCTL)) in a 30 μL total volume. The mice were intraperitoneally administered with PBS containing 1 mM of MgCl 2 (Vehicle; Control (CTL)), 100 mg/kg vitamin C, 2 mg/kg aptamer, or a combination of vitamin C (100 mg/kg) and aptamer (2 mg/kg; Aptamin C). ( A ) Body weight change was recorded three times per week. ( B ) Mortality was monitored daily for 38 days and the percent survival rate was expressed as Kaplan–Meier survival plots. The survival plots were analyzed using a log-rank (Mantel–Cox) test. * p < 0.05 compared to the control.

Article Snippet: A single-stranded DNA (ssDNA) aptamer that selectively binds to vitamin C was obtained from Nexmos Inc.

Techniques: Injection, Negative Control, Control

Journal: Pharmaceuticals

Article Title: Anti-Inflammatory Effects of Aptamin C in Pulmonary Fibrosis Induced by Bleomycin

doi: 10.3390/ph17121577

Figure Lengend Snippet: Reduced infiltration of inflammatory cells in the lungs of Aptamin C-treated mice. Gulo KO mice ( n = 5) injected with bleomycin intraperitoneally were administered PBS containing 1 mM of MgCl 2 (Vehicle; Control (CTL)), 100 mg/kg vitamin C, 2 mg/kg aptamer, or a combination of Vitamin C (100 mg/kg) and aptamer (2 mg/kg; Aptamin C) for 14 days. BALF was obtained by intratracheal injection of 0.8 mL PBS, followed by withdrawal twice. ( A ) The total number of BALF cells was measured using a hemocytometer following staining with trypan blue. Data are presented as the mean ± SEM. * p < 0.05, *** p < 0.001. ( B ) Representative images are shown from Wright–Giemsa stained immune cells in BALF. Photomicrographs were captured at 200× and 400× magnification (scale bar = 150 and 300 µm, respectively). Negative control, NCTL.

Article Snippet: A single-stranded DNA (ssDNA) aptamer that selectively binds to vitamin C was obtained from Nexmos Inc.

Techniques: Injection, Control, Staining, Negative Control

Journal: Pharmaceuticals

Article Title: Anti-Inflammatory Effects of Aptamin C in Pulmonary Fibrosis Induced by Bleomycin

doi: 10.3390/ph17121577

Figure Lengend Snippet: Protective effect of Aptamin C on bleomycin-induced pulmonary fibrosis. Gulo KO mice ( n = 5) were treated with vehicle, vitamin C, aptamer, and Aptamin C for 14 days after bleomycin instillation. The mice were sacrificed, and lung tissue excised. The lung tissue sections were prepared and stained with ( A ) hematoxylin and eosin (H&E) staining and ( B ) Masson’s trichome staining. Photomicrographs were captured at 200× magnification (Scale bar = 150 µm). ( C ) The lung tissues index (tissue weight/body weight) was calculated. ( D ) The severity of lung fibrosis was assessed using the Ashcroft score based on H&E staining. ( E ) Collagen-positive areas (stained blue) based on Masson’s trichrome staining were quantified using Celleste™ image analysis software. A one-way ANOVA with Tukey’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant. Negative control, NCTL; Control, CTL.

Article Snippet: A single-stranded DNA (ssDNA) aptamer that selectively binds to vitamin C was obtained from Nexmos Inc.

Techniques: Staining, Software, Negative Control, Control

Journal: bioRxiv

Article Title: Selection of streptococcal glucan-binding protein C specific DNA aptamers to inhibit biofilm formation

doi: 10.1101/2024.08.23.608024

Figure Lengend Snippet:

Article Snippet: The single-stranded DNA aptamer library was obtained from Sigma.

Techniques: